Setting an oven to high broil for 20-30 minutes will clean the air inside the oven. This makeshift sterile field works well for capturing cultures in a backcountry setting, but it is impractical for most other techniques. When a Bunsen burner or propane torch is pointed upward, a convection current forms that pushes air-born competitors up and away from the area directly below a flame, creating a small sterile field. Several types of transfer spaces are listed below in order of lowest to highest cost and ease of use. Clean transfer spaces ensure the lowest rates of contamination as they minimize the movement of airborne competitors into the sterile interior or substrate containers. Often this is done in a dedicated clean room and/or at a small station.
For several cultivation protocols, mycelium must be moved from one container to another under aseptic conditions. Before you begin cultivating, I strongly recommend getting each of the following workspaces properly set up and to acquire all the materials needed for your desired techniques. Large farms designate discrete rooms for each work area while many home cultivators place all of these spaces together in a garage, shed, or large closet.Īs with any trade, access to a well-designed workspace heavily influences one’s excitement in honing that trade. The size and proximity of these spaces is dependent on the requirements and limitations of a given project, its location, and the relationship of these spaces to each other. At the minimum, a typical aseptic mushroom operation requires three designated areas for the major stages of cultivation: a clean area where mycelium is transferred, an incubation area where the fungi myceliate their substrates, and a humid environment for fruiting mushrooms.
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